In-vitro propagation of Senna alata (linn) on WPM and MS media with varying PGR

Plants react differently to various media concentration and constituents. Growth of Senna alata (linn) inoculated on woody plant media (WPM) and Murashige & Skoog (MS) supplemented with growth regulators were investigated. Mature seeds were collected from the medicinal garden of National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan; latitude 70221N; longitude 30 551E. Seeds of the species were inoculated into WPM and MS media. After 3 weeks, proliferated shoots were excised and inoculated into WPM and MS supplemented with the following concentrations of growth regulators 0.05, 0.075, 0.10, 0.125, 0.15mg/L BAP; 0.05, 0.075, 0.10, 0.125, 0.15mg/L KIN and 0.01mg/L NAA. Within 14 weeks of culture, shoot elongation was observed. Well rooted plantlets were transplanted and acclimatized in the screen house where 75% plantlets survived and grew successfully. The highest mean values of 3.90cm±0.15, 1.37±0.08 and 1.79cm±0.17 for shoot length, shoot number and root length respectively were obtained in plantlets inoculated in WPM supplemented with BAP 0.075mg/L+ NAA 0.01mg/L. Highest mean values of 2.81±0.08 for number of nodes, 2.29±0.15 for root number and 12.78±0.24 for number of leaves were obtained in plantlets inoculated in WPM supplemented with BAP 0.10mg/L+ NAA 0.01mg/L; KIN 0.15mg/L+ NAA0.01mg/L and BAP 0.05mg/L+ NAA 0.01mg/L respectively. The lowest mean values of 0.24±0.04 and 0.46±0.08 were obtained for numbers of nodes and leaves respectively under pure MS (control). Propagation of Senna alata (linn) was most effective with WPM supplemented with BAP 0.05mg/L + NAA 0.01mg/L.